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Topic 8 of 8 · Form 6

Classification, biodiversity, conservation and genetic technology

Three domains (Bacteria, Archaea, Eukarya) and kingdoms; taxonomic hierarchy; viruses classification.

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Classification

Three domains (Bacteria, Archaea, Eukarya) and kingdoms; taxonomic hierarchy; viruses classification.

Biodiversity

Species diversity, genetic diversity and ecosystem diversity; sampling (quadrats, transects, mark–release–recapture); Simpson's index of diversity D = 1 − Σ(n/N)²; correlation (Spearman's rank and Pearson's).

Worked example: A sample contains three species with 10, 20 and 10 individuals. Calculate Simpson's index.

N = 40 → Σ(n/N)² = 0.0625 + 0.25 + 0.0625 = 0.375 → D = 0.625

Conservation

Threats (habitat loss, overexploitation, climate change, invasive species); national parks, zoos, seed banks, assisted reproduction, CITES; community conservation.

Genetic technology

Restriction enzymes, ligase, plasmid vectors, PCR, gel electrophoresis, DNA sequencing, genetically modified organisms (e.g. Bt crops, GM insulin-producing bacteria), gene therapy, genetic screening and ethical issues.

Exam tip: For genetic technology, describe each step in order and name the enzyme used at each step.

Check yourself

  1. Name the three domains of life.
  2. What is the role of DNA ligase?
  3. Give one advantage and one concern about GM crops.
Show answers
  1. Bacteria, Archaea and Eukarya.
  2. To join DNA fragments by forming phosphodiester bonds.
  3. Advantage: pest resistance/higher yield; concern: gene flow to wild plants, loss of biodiversity, cost to farmers.
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